Polymerase variants
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United States of America Patent
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Nov 10, 2020
Grant Date -
Apr 5, 2018
app pub date -
Sep 20, 2017
filing date -
Sep 22, 2016
priority date (Note) -
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Abstract
The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.
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