Arrays of discrete cell culture microenvironments, methods of making such arrays and uses thereof
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United States of America Patent
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Jul 28, 2020
Grant Date -
Apr 26, 2018
app pub date -
Aug 21, 2017
filing date -
May 8, 2013
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Abstract
A method of making an array having discrete volumes of cell culture micro-environments possessing different properties influencing the behavior of encapsulated cells, in particular proliferation, colony-formation, differentiation, migration, or combinations thereof. The method comprises a) providing different hydrogel precursor molecules, where the precursor molecules are linear or branched hydrophilic polymers for building up cell culture micro-environments; b) combining and dispensing different combinations of the hydrogel precursor molecules, according to step a), onto discrete volumes of a multi-well plate; c) adding one or more biologically active molecules to the discrete volumes and either attaching the molecules to one of the hydrogel precursor molecules present or a hydrogel, formed in step e), or allowing them to diffuse freely; d) adding cells onto/into the discrete volumes of the multi-well plate; and e) crosslinking the hydrogel precursor molecules based on an enzymatically catalyzed reaction or a Michael addition reaction to form a hydrogel matrix.
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