Selective plane illumination microscopy (SPIM) systems and methods
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United States of America Patent
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Apr 14, 2020
Grant Date -
Mar 21, 2019
app pub date -
Nov 8, 2018
filing date -
Oct 2, 2014
priority date (Note) -
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status (Latency Note)
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Abstract
In one embodiment, a selective plane illumination microscopy system for capturing light emitted by an illuminated specimen, the system including a specimen support having a top surface configured to support a specimen holder and an opening configured to provide access to a bottom of the specimen holder, and a selective plane illumination microscopy optical system positioned beneath the specimen support, the optical system configured to illuminate the specimen with a sheet of excitation light and including an excitation objective, a detection objective, and an open-top, hollow imaging element that is configured to contain a liquid, wherein the imaging element is positioned within the opening of the specimen support and optical axes of the objectives are aligned with the imaging element such that the axes pass through the imaging element and intersect at a position near the top surface of the specimen support.
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