Compounds and methods for CRISPR/Cas-based genome editing by homologous recombination
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United States of America
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Jan 7, 2020
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Mar 2, 2017
app pub date -
Aug 30, 2016
filing date -
Aug 30, 2016
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Abstract
The present invention relates to guide RNAs comprising adaptor segments having one or more modifications, and their use in homologous recombination by CRISPR:Cas systems. The modified adaptor segments are resistant to degradation by RNaseH. The present invention also relates to a dual guide RNA strategy in which a first guide RNA directs a Cas enzyme to make a double-strand break at a first target sequence, and a second guide RNA comprises an adaptor segment attached to a donor polynucleotide, and binds a second target sequence that is offset from the first target sequence.
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