Optical selection of cells
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United States of America Patent
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Sep 18, 2018
Grant Date -
Jul 21, 2016
app pub date -
Jan 14, 2016
filing date -
Jan 15, 2015
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Abstract
Provided herein are photochemical crosslinkers and photocleavable crosslinkers and their uses in methods for cell selection from cell cultures. The photochemical crosslinkers comprise a fluorescent dye and a radical generator. The photocleavable crosslinkers comprise a photocleavable linker linking two electrophilic groups to each other. Also provided are systems for imaging cells comprising a plurality of cells crosslinked to extracellular matrix proteins using a crosslinker as described, an imaging apparatus, an illuminating apparatus, and software for image processing.
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