Convex lens-induced confinement for measuring distributions of molecular size
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United States of America Patent
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Aug 14, 2018
Grant Date -
Jun 8, 2017
app pub date -
Aug 11, 2016
filing date -
Jan 15, 2010
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Abstract
A curved surface is placed tangent to a slide and displaces a sample liquid from the point or line of contact outward. Imaging indicates a region where fluorescence is observed, and the location of the fluorescence indicates the molecular size. The radius of curvature of the lens is known, the distance from the (center) point of contact of the observed fluorescence is measured with a microscope and the distance of the lens surface to the slide's surface can then be calculated. This distance represents the size of the molecule or ensemble of molecules emitting. Similarly, absorbance, etc. could be measured with a light source below the slide.
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